The Vector Core Laboratory was established in March of 1995 when the first of two laboratory modules targeted for the Vector Core became available in the Academic Research Building (ARB). In September of 1995, the Vector Core Laboratory occupied a second laboratory module in the ARB to reach its current size of approximately 1500 sq.ft. In its first year of operation, Sergei Zolotukhin, Ph.D., the previous Director, had equipped the first module for wet lab DNA manipulation and cloning and the second module as a tissue culture facility for producing preclinical vectors and for training staff from other laboratories. The current facility director is Sanford Boye.
The primary role of the Vector Core Laboratory is to provide the service of generating gene therapy vectors. This service is comprehensive, starting with consultation on construct design and AAV capsid selection, molecular cloning of AAV plasmids, and finally packaging and purification of the virus. The Vector Core has served this role for a number of NIH program project grants worth over $25M and produces over 400 preps per year for both academic and industry sponsors. In addition, the Vector Core serves a training role for students associated with those programs.
Vector Core assists with developing new vector technology in collaboration with Center faculty. As such Vector Core has access to many of the new AAV capsids developed at the University of Florida.
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AAV Vector Core Auxiliary Direct Cost List
|Plasmid Transformation||Incoming DNA transformed into competent cells, characterized and stored.||$268.00|
|Plasmid Preparation for AAV Production||4LPlasmid DNA Preparation by CsCl and characterization||$442.00|
|Plasmid Preparation for AAV Production||2.5L Plasmid DNA Preparation by EndoFree Qiagen Mega Kit and characterization||$353.00|
|AAV Vector Preparation||Transfection of ONE CellStack, cell culture, purification by iodixanol and/or columns, concentration, vialing, storage||$2,630.00|
|AAV Vector Preparation||Each Additional CS pooled with the first CS (up to 4 addtn’)||$1,340.00|
|AAV Vector Preparation Characterization (QC)||Purity/Identity by Imperial Blue stain PAGE (per preparation)||$70.00|
|AAV Vector Preparation Characterization (QC)||Vector Genome Titer by qPCR (per preparation)||$90.00|
|Controls||AAV-Marker control preparation @ 25uL||$75.00|
|Controls||AAV-Marker control preparation @ 100uL||$300.00|
|AAV Vector Preparation – Small Scale||Transfection of 5 Triple Flasks, Harvest, purification by Iodixanol only, concentration, vialing||$875.00|
|Optional AAV Preparation QC Testing||Vector Genome Titer by Q-PCR (CMV, CBA, SV40polyA)(per preparation)||$388.00|
|Endotoxin Testing (optional)||Determination of level of endotoxins in plasmids and AAV preparations (per preparation)||$100.00|