We offer a full service, semi-customizable AAV Vector production service. With vector production, we provide consultation regarding capsid selection as well as gene transfer plasmid design. Our standard vector production platform uses adherent HEK293 cells grown in a Corning 10-layer cell stack (6,360cm2 cell growth area). Production scale is achieved using multiple cell stacks and batching of the final material. We use standard industry methods for AAV Vector purification resulting in vector preps having 50-90% full capsids. The purity of our vector preps at completion is sufficient for use in most animal research and in vitro studies. In the event that non-human primates (NHP) are used in your research, or the highest level of purity is desired, we offer additional purification using FPLC affinity chromatography.
We have the ability to customize and modify each step of the production process to meet the individual needs of your vector production requirements. These can include ultra-purification using Cesium Chloride, yielding nearly complete full capsid production, production of empty capsid, generating low level purification material for use in feeding midstream processing. We offer both qPCR and ddPCR tittering depending on client preference.
Request a Quote
With just a few questions regarding your AAV vector production interests, we can generate a basic quote for your project. This will also open a pathway for any further questions that you may have regarding the production of your AAV vector.