Quote Generator | Industry "*" indicates required fields 1AAV Plasmid Production2AAV Plasmid Cloning3AAV Vector Production4Contact Information AAV Plasmid Production 2 Liter Plasmid DNA prep using EndoFree Qiagen Mega kit and characterization. Physical turnaround time is 2-4 weeksWould you like to order a Vector Plasmid Production?Please note you can order up to 5 different plasmids Yes No Vector Plasmid #1Plasmid Order #1 Price: Enter the Plasmid Name Specify Plasmid Type AAV vector plasmid (AAV ITR cassette) AAV rep-cap plasmid Would you like to order a SECOND plasmid? Yes No Vector Plasmid #2Plasmid Order #2 Price: Enter the name of your SECOND plasmid Specify Plasmid Type AAV vector plasmid (AAV ITR cassette) AAV rep-cap plasmid Would you like to order a THIRD plasmid? Yes No Vector Plasmid #3Plasmid Order #3 Price: Enter the name of your THIRD plasmid Specify Plasmid Type AAV vector plasmid (AAV ITR cassette) AAV rep-cap plasmid Would you like to order a FOURTH plasmid? Yes No Vector Plasmid #4Plasmid Order #4 Price: Enter the name of your FOURTH plasmid Specify Plasmid Type AAV vector plasmid (AAV ITR cassette) AAV rep-cap plasmid Would you like to order a FIFTH plasmid? Yes No Vector Plasmid #5Plasmid Order #5 Price: Enter the name of your FIFTH plasmid Specify Plasmid Type AAV vector plasmid (AAV ITR cassette) AAV rep-cap plasmid Running Total AAV Plasmid Cloning We will clone the cDNA of your interest into either a Constitutive Promoter Constructs or a Specific Tissue. Please allow 4-8 weeks for completion of cloningWould you like to order the cloning of a custom AAV Plasmid?Please note you can order up to 5 different clones Yes No Plasmid Clone #1Cloning #1 Price: Note: This price does not include the cost of the plasmid producation (Additional fees may apply depending on the cost of the custom cDNA synthesis by Genscript, or the cDNA provided by investigator.) Enter Name of cDNA Enter cDNA Sequence Chose a Promoter Construct Constitutive - CMV immediate early promoter (CMV) Constitutive - Chimeric CMV-Chicken beta-actin promoter (CBA) Constitutive - Chimeric CMV-Chicken beta-actin promoter (CBA) with WPRE Constitutive - Small Chimeric CMV-Chicken beta-actin promoter (smCBA) Tissue Specific Retina and neuronal - Human rhodopsin kinase promoter (GRK1) Tissue Specific Retina and neuronal - Human synapsin promoter (Syn) Would you like to order a SECOND clone? Yes No Plasmid Clone #2Cloning #2 Price: Note: This price does not include the cost of the plasmid producation (Additional fees may apply depending on the cost of the custom cDNA synthesis by Genscript, or the cDNA provided by investigator.) Enter Name of SECOND cDNA Enter SECOND cDNA Sequence Chose a Promoter Construct Constitutive - CMV immediate early promoter (CMV) Constitutive - Chimeric CMV-Chicken beta-actin promoter (CBA) Constitutive - Chimeric CMV-Chicken beta-actin promoter (CBA) with WPRE Constitutive - Small Chimeric CMV-Chicken beta-actin promoter (smCBA) Tissue Specific Retina and neuronal - Human rhodopsin kinase promoter (GRK1) Tissue Specific Retina and neuronal - Human synapsin promoter (Syn) Would you like to order a THIRD clone? Yes No Plasmid Clone #3Cloning #3 Price: Note: This price does not include the cost of the plasmid producation (Additional fees may apply depending on the cost of the custom cDNA synthesis by Genscript, or the cDNA provided by investigator.) Enter Name of THIRD cDNA Enter THIRD cDNA Sequence Chose a Promoter Construct Constitutive - CMV immediate early promoter (CMV) Constitutive - Chimeric CMV-Chicken beta-actin promoter (CBA) Constitutive - Chimeric CMV-Chicken beta-actin promoter (CBA) with WPRE Constitutive - Small Chimeric CMV-Chicken beta-actin promoter (smCBA) Tissue Specific Retina and neuronal - Human rhodopsin kinase promoter (GRK1) Tissue Specific Retina and neuronal - Human synapsin promoter (Syn) Would you like to order a FORTH clone? Yes No Plasmid Clone #4Cloning #4 Price: Note: This price does not include the cost of the plasmid producation (Additional fees may apply depending on the cost of the custom cDNA synthesis by Genscript, or the cDNA provided by investigator.) Enter Name of FOURTH cDNA Enter FOURTH cDNA Sequence Chose a Promoter Construct Constitutive - CMV immediate early promoter (CMV) Constitutive - Chimeric CMV-Chicken beta-actin promoter (CBA) Constitutive - Chimeric CMV-Chicken beta-actin promoter (CBA) with WPRE Constitutive - Small Chimeric CMV-Chicken beta-actin promoter (smCBA) Tissue Specific Retina and neuronal - Human rhodopsin kinase promoter (GRK1) Tissue Specific Retina and neuronal - Human synapsin promoter (Syn) Would you like to order a FIFTH clone? Yes No Plasmid Clone #5Cloning #5 Price: Note: This price does not include the cost of the plasmid producation (Additional fees may apply depending on the cost of the custom cDNA synthesis by Genscript, or the cDNA provided by investigator.) Enter Name of FIFTH cDNA Enter FIFTH cDNA Sequence Chose a Promoter Construct Constitutive - CMV immediate early promoter (CMV) Constitutive - Chimeric CMV-Chicken beta-actin promoter (CBA) Constitutive - Chimeric CMV-Chicken beta-actin promoter (CBA) with WPRE Constitutive - Small Chimeric CMV-Chicken beta-actin promoter (smCBA) Tissue Specific Retina and neuronal - Human rhodopsin kinase promoter (GRK1) Tissue Specific Retina and neuronal - Human synapsin promoter (Syn) Running Total AAV Vector ProductionWould you like to order Vector Production?Note: Individual quotes limited to a total of 10 AAV vectors. If you have additional vectors to order please complete a second quote request or contact vectorcore-quoterequest@peds.ufl.edu Yes No Vector Production #1AAV Capsid Selection Price: Includes transfection of a 10 layer cell stack of HEK293 cells, purification of AAV by iodixanol gradient, ion exchange chromatography (if applicable capsid type) and buffer exchange/concentration of final vector into user defined buffer and testing of final AAV vector for vector genome titer (qPCR) and purity/identity (PAGE). Typical yield of AAV is 5e12vg to 5e13vg per cell stack, and titers of 5e12vg/ml to 1e14 vg/ml, depending on both the capsid and vector cassette packaged. Cost includes AAV cap plasmid (unless novel capsid, then plasmid supplied by client) and pAd helper plasmid. Typical turnaround for vector modification is 4-6 weeks from receipt of client plasmidSelect Capsid: AAV1 AAV2 AAV2(Y444F) AAV2-TrpYF, aka Y444F+Y500F+Y730F AAV2(quadY-F+T-V), aka Y272F+Y444F+Y500F+Y730F+T491V AAV2(4pMut)ΔHS AAV5 AAV6 AAV6-TrpMut, aka Y705F+Y731F+T492V AAV8 AAV8(Y733F) AAV8-TrpMut, aka Y447F+Y733F+T494V AAV9 AAV44.9 AAV44.9 (E531D) Select AAV Vector Plasmid Vector Core reporter constructs Client provided AAV vector plasmid Select Reporter Construct pTR-UF5 (CMV-GFP) pTR-UF11 (CBA-GFP) CBA-mCherry with WPRE CBA-Luciferase(ff) Self-complementary CBA-mCherry Self-complementary CBA-GFP Note: we require 650ug of AAV vector plasmid DNA per Cell StackName of AAV Vector Plasmid Gene of Interest Select how many Cell Stacks you would likeFirst Cell Stack is $2,630. Preparations requiring up to 4 additional Cell Stacks are discounted at a rate of $1,742 per additional Cell Stack. Please contact the Vector Core if you suspect your request will exceed a total of 5 Cell Stacks. If ordering more than one cell stack, please note that they will be pooled together.1 Cell stack2 Cell stacks3 Cell stacks4 Cell stacks5 Cell stacksSelect Additional AAV Purification (Optional) - $700 None Affinity purification with AAVX Affinity purification with CaptureSelect AAV8 Affinity purification with CaptureSelect AAV9 *Please inquire if additional processing for enhanced for full capsids is desired. (sboye@ufl.edu) Select AAV BufferUnless specified, our final excipient buffers will contain 0.001% Pluronic F68. Pluronic is a widely used non-ionic surfactant added to prevent aggregation during concentration and storage. *Deviations from standard buffers are acceptable but may require a brief consult. Balanced Salt Solution plus Pluronic (BSS-P) Lactated Ringers Solution plus Pluronic (LRS-P) TMN200-P Phosphate Buffered Saline plus Pluronic (PBS-P) Other Please specify the AAV Buffer you would likeCompatibility of the buffers listed has been confirmed, selection of an alternate buffer is at the requester's risk Select Additional Testing of Final AAV Vector Endotoxin testing (with Charles River Endosafe-PTS) Please include any special requests or additional commentsWould you like to order a SECOND vector? Yes No Vector Production #2AAV Capsid Selection Price: Includes transfection of a 10 layer cell stack of HEK293 cells, purification of AAV by iodixanol gradient, ion exchange chromatography (if applicable capsid type) and buffer exchange/concentration of final vector into user defined buffer and testing of final AAV vector for vector genome titer (qPCR) and purity/identity (PAGE). Typical yield of AAV is 5e12vg to 5e13vg per cell stack, and titers of 5e12vg/ml to 1e14 vg/ml, depending on both the capsid and vector cassette packaged. Cost includes AAV cap plasmid (unless novel capsid, then plasmid supplied by client) and pAd helper plasmid. Typical turnaround for vector modification is 4-6 weeks from receipt of client plasmidSelect Capsid: AAV1 AAV2 AAV2(Y444F) AAV2-TrpYF, aka Y444F+Y500F+Y730F AAV2(quadY-F+T-V), aka Y272F+Y444F+Y500F+Y730F+T491V AAV2(4pMut)ΔHS AAV5 AAV6 AAV6-TrpMut, aka Y705F+Y731F+T492V AAV8 AAV8(Y733F) AAV8-TrpMut, aka Y447F+Y733F+T494V AAV9 AAV44.9 AAV44.9 (E531D) Select AAV Vector Plasmid Vector Core reporter constructs Client provided AAV vector plasmid Select Reporter Construct pTR-UF5 (CMV-GFP) pTR-UF11 (CBA-GFP) CBA-mCherry with WPRE CBA-Luciferase(ff) Self-complementary CBA-mCherry Self-complementary CBA-GFP Note: we require 650ug of AAV vector plasmid DNA per Cell StackName of AAV Vector Plasmid Gene of Interest Select how many Cell Stacks you would likeFirst Cell Stack is $2,630. Preparations requiring up to 4 additional Cell Stacks are discounted at a rate of $1,742 per additional Cell Stack. Please contact the Vector Core if you suspect your request will exceed a total of 5 Cell Stacks. If ordering more than one cell stack, please note that they will be pooled together.1 Cell stack2 Cell stacks3 Cell stacks4 Cell stacks5 Cell stacksSelect Additional AAV Purification (Optional) - $700 None Affinity purification with AAVX Affinity purification with CaptureSelect AAV8 Affinity purification with CaptureSelect AAV9 *Please inquire if additional processing for enhanced for full capsids is desired. (sboye@ufl.edu) Select AAV BufferUnless specified, our final excipient buffers will contain 0.001% Pluronic F68. Pluronic is a widely used non-ionic surfactant added to prevent aggregation during concentration and storage. *Deviations from standard buffers are acceptable but may require a brief consult. Balanced Salt Solution plus Pluronic (BSS-P) Lactated Ringers Solution plus Pluronic (LRS-P) TMN200-P Phosphate Buffered Saline plus Pluronic (PBS-P) Other Please specify the AAV Buffer you would likeCompatibility of the buffers listed has been confirmed, selection of an alternate buffer is at the requester's risk Select Additional Testing of Final AAV Vector Endotoxin testing (with Charles River Endosafe-PTS) Please include any special requests or additional commentsWould you like to order a THIRD vector? Yes No Vector Production #3AAV Capsid Selection Price: Includes transfection of a 10 layer cell stack of HEK293 cells, purification of AAV by iodixanol gradient, ion exchange chromatography (if applicable capsid type) and buffer exchange/concentration of final vector into user defined buffer and testing of final AAV vector for vector genome titer (qPCR) and purity/identity (PAGE). Typical yield of AAV is 5e12vg to 5e13vg per cell stack, and titers of 5e12vg/ml to 1e14 vg/ml, depending on both the capsid and vector cassette packaged. Cost includes AAV cap plasmid (unless novel capsid, then plasmid supplied by client) and pAd helper plasmid. Typical turnaround for vector modification is 4-6 weeks from receipt of client plasmidSelect Capsid: AAV1 AAV2 AAV2(Y444F) AAV2-TrpYF, aka Y444F+Y500F+Y730F AAV2(quadY-F+T-V), aka Y272F+Y444F+Y500F+Y730F+T491V AAV2(4pMut)ΔHS AAV5 AAV6 AAV6-TrpMut, aka Y705F+Y731F+T492V AAV8 AAV8(Y733F) AAV8-TrpMut, aka Y447F+Y733F+T494V AAV9 AAV44.9 AAV44.9 (E531D) Select AAV Vector Plasmid Vector Core reporter constructs Client provided AAV vector plasmid Select Reporter Construct pTR-UF5 (CMV-GFP) pTR-UF11 (CBA-GFP) CBA-mCherry with WPRE CBA-Luciferase(ff) Self-complementary CBA-mCherry Self-complementary CBA-GFP Note: we require 650ug of AAV vector plasmid DNA per Cell StackName of AAV Vector Plasmid Gene of Interest Select how many Cell Stacks you would likeFirst Cell Stack is $2,630. Preparations requiring up to 4 additional Cell Stacks are discounted at a rate of $1,742 per additional Cell Stack. Please contact the Vector Core if you suspect your request will exceed a total of 5 Cell Stacks. If ordering more than one cell stack, please note that they will be pooled together.1 Cell stack2 Cell stacks3 Cell stacks4 Cell stacks5 Cell stacksSelect Additional AAV Purification (Optional) - $700 None Affinity purification with AAVX Affinity purification with CaptureSelect AAV8 Affinity purification with CaptureSelect AAV9 *Please inquire if additional processing for enhanced for full capsids is desired. (sboye@ufl.edu) Select AAV BufferUnless specified, our final excipient buffers will contain 0.001% Pluronic F68. Pluronic is a widely used non-ionic surfactant added to prevent aggregation during concentration and storage. *Deviations from standard buffers are acceptable but may require a brief consult. Balanced Salt Solution plus Pluronic (BSS-P) Lactated Ringers Solution plus Pluronic (LRS-P) TMN200-P Phosphate Buffered Saline plus Pluronic (PBS-P) Other Please specify the AAV Buffer you would likeCompatibility of the buffers listed has been confirmed, selection of an alternate buffer is at the requester's risk Select Additional Testing of Final AAV Vector Endotoxin testing (with Charles River Endosafe-PTS) Please include any special requests or additional commentsWould you like to order a FOURTH vector? Yes No Vector Production #4AAV Capsid Selection Price: Includes transfection of a 10 layer cell stack of HEK293 cells, purification of AAV by iodixanol gradient, ion exchange chromatography (if applicable capsid type) and buffer exchange/concentration of final vector into user defined buffer and testing of final AAV vector for vector genome titer (qPCR) and purity/identity (PAGE). Typical yield of AAV is 5e12vg to 5e13vg per cell stack, and titers of 5e12vg/ml to 1e14 vg/ml, depending on both the capsid and vector cassette packaged. Cost includes AAV cap plasmid (unless novel capsid, then plasmid supplied by client) and pAd helper plasmid. Typical turnaround for vector modification is 4-6 weeks from receipt of client plasmidSelect Capsid: AAV1 AAV2 AAV2(Y444F) AAV2-TrpYF, aka Y444F+Y500F+Y730F AAV2(quadY-F+T-V), aka Y272F+Y444F+Y500F+Y730F+T491V AAV2(4pMut)ΔHS AAV5 AAV6 AAV6-TrpMut, aka Y705F+Y731F+T492V AAV8 AAV8(Y733F) AAV8-TrpMut, aka Y447F+Y733F+T494V AAV9 AAV44.9 AAV44.9 (E531D) Select AAV Vector Plasmid Vector Core reporter constructs Client provided AAV vector plasmid Select Reporter Construct pTR-UF5 (CMV-GFP) pTR-UF11 (CBA-GFP) CBA-mCherry with WPRE CBA-Luciferase(ff) Self-complementary CBA-mCherry Self-complementary CBA-GFP Note: we require 650ug of AAV vector plasmid DNA per Cell StackName of AAV Vector Plasmid Gene of Interest Select how many Cell Stacks you would likeFirst Cell Stack is $2,630. Preparations requiring up to 4 additional Cell Stacks are discounted at a rate of $1,742 per additional Cell Stack. Please contact the Vector Core if you suspect your request will exceed a total of 5 Cell Stacks. If ordering more than one cell stack, please note that they will be pooled together.1 Cell stack2 Cell stacks3 Cell stacks4 Cell stacks5 Cell stacksSelect Additional AAV Purification (Optional) - $700 None Affinity purification with AAVX Affinity purification with CaptureSelect AAV8 Affinity purification with CaptureSelect AAV9 *Please inquire if additional processing for enhanced for full capsids is desired. (sboye@ufl.edu) Select AAV BufferUnless specified, our final excipient buffers will contain 0.001% Pluronic F68. Pluronic is a widely used non-ionic surfactant added to prevent aggregation during concentration and storage. *Deviations from standard buffers are acceptable but may require a brief consult. Balanced Salt Solution plus Pluronic (BSS-P) Lactated Ringers Solution plus Pluronic (LRS-P) TMN200-P Phosphate Buffered Saline plus Pluronic (PBS-P) Other Please specify the AAV Buffer you would likeCompatibility of the buffers listed has been confirmed, selection of an alternate buffer is at the requester's risk Select Additional Testing of Final AAV Vector Endotoxin testing (with Charles River Endosafe-PTS) Please include any special requests or additional commentsWould you like to order a FIFTH vector? Yes No Vector Production #5AAV Capsid Selection Price: Includes transfection of a 10 layer cell stack of HEK293 cells, purification of AAV by iodixanol gradient, ion exchange chromatography (if applicable capsid type) and buffer exchange/concentration of final vector into user defined buffer and testing of final AAV vector for vector genome titer (qPCR) and purity/identity (PAGE). Typical yield of AAV is 5e12vg to 5e13vg per cell stack, and titers of 5e12vg/ml to 1e14 vg/ml, depending on both the capsid and vector cassette packaged. Cost includes AAV cap plasmid (unless novel capsid, then plasmid supplied by client) and pAd helper plasmid. Typical turnaround for vector modification is 4-6 weeks from receipt of client plasmidSelect Capsid: AAV1 AAV2 AAV2(Y444F) AAV2-TrpYF, aka Y444F+Y500F+Y730F AAV2(quadY-F+T-V), aka Y272F+Y444F+Y500F+Y730F+T491V AAV2(4pMut)ΔHS AAV5 AAV6 AAV6-TrpMut, aka Y705F+Y731F+T492V AAV8 AAV8(Y733F) AAV8-TrpMut, aka Y447F+Y733F+T494V AAV9 AAV44.9 AAV44.9 (E531D) Select AAV Vector Plasmid Vector Core reporter constructs Client provided AAV vector plasmid Select Reporter Construct pTR-UF5 (CMV-GFP) pTR-UF11 (CBA-GFP) CBA-mCherry with WPRE CBA-Luciferase(ff) Self-complementary CBA-mCherry Self-complementary CBA-GFP Note: we require 650ug of AAV vector plasmid DNA per Cell StackName of AAV Vector Plasmid Gene of Interest Select how many Cell Stacks you would likeFirst Cell Stack is $2,630. Preparations requiring up to 4 additional Cell Stacks are discounted at a rate of $1,742 per additional Cell Stack. Please contact the Vector Core if you suspect your request will exceed a total of 5 Cell Stacks. If ordering more than one cell stack, please note that they will be pooled together.1 Cell stack2 Cell stacks3 Cell stacks4 Cell stacks5 Cell stacksSelect Additional AAV Purification (Optional) - $700 None Affinity purification with AAVX Affinity purification with CaptureSelect AAV8 Affinity purification with CaptureSelect AAV9 *Please inquire if additional processing for enhanced for full capsids is desired. (sboye@ufl.edu) Select AAV BufferUnless specified, our final excipient buffers will contain 0.001% Pluronic F68. Pluronic is a widely used non-ionic surfactant added to prevent aggregation during concentration and storage. *Deviations from standard buffers are acceptable but may require a brief consult. Balanced Salt Solution plus Pluronic (BSS-P) Lactated Ringers Solution plus Pluronic (LRS-P) TMN200-P Phosphate Buffered Saline plus Pluronic (PBS-P) Other Please specify the AAV Buffer you would likeCompatibility of the buffers listed has been confirmed, selection of an alternate buffer is at the requester's risk Select Additional Testing of Final AAV Vector Endotoxin testing (with Charles River Endosafe-PTS) Please include any special requests or additional commentsWould you like to order a SIXTH vector? Yes No Vector Production #6AAV Capsid Selection Price: Includes transfection of a 10 layer cell stack of HEK293 cells, purification of AAV by iodixanol gradient, ion exchange chromatography (if applicable capsid type) and buffer exchange/concentration of final vector into user defined buffer and testing of final AAV vector for vector genome titer (qPCR) and purity/identity (PAGE). Typical yield of AAV is 5e12vg to 5e13vg per cell stack, and titers of 5e12vg/ml to 1e14 vg/ml, depending on both the capsid and vector cassette packaged. Cost includes AAV cap plasmid (unless novel capsid, then plasmid supplied by client) and pAd helper plasmid. Typical turnaround for vector modification is 4-6 weeks from receipt of client plasmidSelect Capsid: AAV1 AAV2 AAV2(Y444F) AAV2-TrpYF, aka Y444F+Y500F+Y730F AAV2(quadY-F+T-V), aka Y272F+Y444F+Y500F+Y730F+T491V AAV2(4pMut)ΔHS AAV5 AAV6 AAV6-TrpMut, aka Y705F+Y731F+T492V AAV8 AAV8(Y733F) AAV8-TrpMut, aka Y447F+Y733F+T494V AAV9 AAV44.9 AAV44.9 (E531D) Select AAV Vector Plasmid Vector Core reporter constructs Client provided AAV vector plasmid Select Reporter Construct pTR-UF5 (CMV-GFP) pTR-UF11 (CBA-GFP) CBA-mCherry with WPRE CBA-Luciferase(ff) Self-complementary CBA-mCherry Self-complementary CBA-GFP Note: we require 650ug of AAV vector plasmid DNA per Cell StackName of AAV Vector Plasmid Gene of Interest Select how many Cell Stacks you would likeFirst Cell Stack is $2,630. Preparations requiring up to 4 additional Cell Stacks are discounted at a rate of $1,742 per additional Cell Stack. Please contact the Vector Core if you suspect your request will exceed a total of 5 Cell Stacks. If ordering more than one cell stack, please note that they will be pooled together.1 Cell stack2 Cell stacks3 Cell stacks4 Cell stacks5 Cell stacksSelect Additional AAV Purification (Optional) - $700 None Affinity purification with AAVX Affinity purification with CaptureSelect AAV8 Affinity purification with CaptureSelect AAV9 *Please inquire if additional processing for enhanced for full capsids is desired. (sboye@ufl.edu) Select AAV BufferUnless specified, our final excipient buffers will contain 0.001% Pluronic F68. Pluronic is a widely used non-ionic surfactant added to prevent aggregation during concentration and storage. *Deviations from standard buffers are acceptable but may require a brief consult. Balanced Salt Solution plus Pluronic (BSS-P) Lactated Ringers Solution plus Pluronic (LRS-P) TMN200-P Phosphate Buffered Saline plus Pluronic (PBS-P) Other Please specify the AAV Buffer you would likeCompatibility of the buffers listed has been confirmed, selection of an alternate buffer is at the requester's risk Select Additional Testing of Final AAV Vector Endotoxin testing (with Charles River Endosafe-PTS) Please include any special requests or additional commentsWould you like to order a SEVENTH vector? Yes No Vector Production #7AAV Capsid Selection Price: Includes transfection of a 10 layer cell stack of HEK293 cells, purification of AAV by iodixanol gradient, ion exchange chromatography (if applicable capsid type) and buffer exchange/concentration of final vector into user defined buffer and testing of final AAV vector for vector genome titer (qPCR) and purity/identity (PAGE). Typical yield of AAV is 5e12vg to 5e13vg per cell stack, and titers of 5e12vg/ml to 1e14 vg/ml, depending on both the capsid and vector cassette packaged. Cost includes AAV cap plasmid (unless novel capsid, then plasmid supplied by client) and pAd helper plasmid. Typical turnaround for vector modification is 4-6 weeks from receipt of client plasmidSelect Capsid: AAV1 AAV2 AAV2(Y444F) AAV2-TrpYF, aka Y444F+Y500F+Y730F AAV2(quadY-F+T-V), aka Y272F+Y444F+Y500F+Y730F+T491V AAV2(4pMut)ΔHS AAV5 AAV6 AAV6-TrpMut, aka Y705F+Y731F+T492V AAV8 AAV8(Y733F) AAV8-TrpMut, aka Y447F+Y733F+T494V AAV9 AAV44.9 AAV44.9 (E531D) Select AAV Vector Plasmid Vector Core reporter constructs Client provided AAV vector plasmid Select Reporter Construct pTR-UF5 (CMV-GFP) pTR-UF11 (CBA-GFP) CBA-mCherry with WPRE CBA-Luciferase(ff) Self-complementary CBA-mCherry Self-complementary CBA-GFP Note: we require 650ug of AAV vector plasmid DNA per Cell StackName of AAV Vector Plasmid Gene of Interest Select how many Cell Stacks you would likeFirst Cell Stack is $2,630. Preparations requiring up to 4 additional Cell Stacks are discounted at a rate of $1,742 per additional Cell Stack. Please contact the Vector Core if you suspect your request will exceed a total of 5 Cell Stacks. If ordering more than one cell stack, please note that they will be pooled together.1 Cell stack2 Cell stacks3 Cell stacks4 Cell stacks5 Cell stacksSelect Additional AAV Purification (Optional) - $700 None Affinity purification with AAVX Affinity purification with CaptureSelect AAV8 Affinity purification with CaptureSelect AAV9 *Please inquire if additional processing for enhanced for full capsids is desired. (sboye@ufl.edu) Select AAV BufferUnless specified, our final excipient buffers will contain 0.001% Pluronic F68. Pluronic is a widely used non-ionic surfactant added to prevent aggregation during concentration and storage. *Deviations from standard buffers are acceptable but may require a brief consult. Balanced Salt Solution plus Pluronic (BSS-P) Lactated Ringers Solution plus Pluronic (LRS-P) TMN200-P Phosphate Buffered Saline plus Pluronic (PBS-P) Other Please specify the AAV Buffer you would likeCompatibility of the buffers listed has been confirmed, selection of an alternate buffer is at the requester's risk Select Additional Testing of Final AAV Vector Endotoxin testing (with Charles River Endosafe-PTS) Please include any special requests or additional commentsWould you like to order a EIGHTH vector? Yes No Vector Production #8AAV Capsid Selection Price: Includes transfection of a 10 layer cell stack of HEK293 cells, purification of AAV by iodixanol gradient, ion exchange chromatography (if applicable capsid type) and buffer exchange/concentration of final vector into user defined buffer and testing of final AAV vector for vector genome titer (qPCR) and purity/identity (PAGE). Typical yield of AAV is 5e12vg to 5e13vg per cell stack, and titers of 5e12vg/ml to 1e14 vg/ml, depending on both the capsid and vector cassette packaged. Cost includes AAV cap plasmid (unless novel capsid, then plasmid supplied by client) and pAd helper plasmid. Typical turnaround for vector modification is 4-6 weeks from receipt of client plasmidSelect Capsid: AAV1 AAV2 AAV2(Y444F) AAV2-TrpYF, aka Y444F+Y500F+Y730F AAV2(quadY-F+T-V), aka Y272F+Y444F+Y500F+Y730F+T491V AAV2(4pMut)ΔHS AAV5 AAV6 AAV6-TrpMut, aka Y705F+Y731F+T492V AAV8 AAV8(Y733F) AAV8-TrpMut, aka Y447F+Y733F+T494V AAV9 AAV44.9 AAV44.9 (E531D) Select AAV Vector Plasmid Vector Core reporter constructs Client provided AAV vector plasmid Select Reporter Construct pTR-UF5 (CMV-GFP) pTR-UF11 (CBA-GFP) CBA-mCherry with WPRE CBA-Luciferase(ff) Self-complementary CBA-mCherry Self-complementary CBA-GFP Note: we require 650ug of AAV vector plasmid DNA per Cell StackName of AAV Vector Plasmid Gene of Interest Select how many Cell Stacks you would likeFirst Cell Stack is $2,630. Preparations requiring up to 4 additional Cell Stacks are discounted at a rate of $1,742 per additional Cell Stack. Please contact the Vector Core if you suspect your request will exceed a total of 5 Cell Stacks. If ordering more than one cell stack, please note that they will be pooled together.1 Cell stack2 Cell stacks3 Cell stacks4 Cell stacks5 Cell stacksSelect Additional AAV Purification (Optional) - $700 None Affinity purification with AAVX Affinity purification with CaptureSelect AAV8 Affinity purification with CaptureSelect AAV9 *Please inquire if additional processing for enhanced for full capsids is desired. (sboye@ufl.edu) Select AAV BufferUnless specified, our final excipient buffers will contain 0.001% Pluronic F68. Pluronic is a widely used non-ionic surfactant added to prevent aggregation during concentration and storage. *Deviations from standard buffers are acceptable but may require a brief consult. Balanced Salt Solution plus Pluronic (BSS-P) Lactated Ringers Solution plus Pluronic (LRS-P) TMN200-P Phosphate Buffered Saline plus Pluronic (PBS-P) Other Please specify the AAV Buffer you would likeCompatibility of the buffers listed has been confirmed, selection of an alternate buffer is at the requester's risk Select Additional Testing of Final AAV Vector Endotoxin testing (with Charles River Endosafe-PTS) Please include any special requests or additional commentsWould you like to order a NINTH vector? Yes No Vector Production #9AAV Capsid Selection Price: Includes transfection of a 10 layer cell stack of HEK293 cells, purification of AAV by iodixanol gradient, ion exchange chromatography (if applicable capsid type) and buffer exchange/concentration of final vector into user defined buffer and testing of final AAV vector for vector genome titer (qPCR) and purity/identity (PAGE). Typical yield of AAV is 5e12vg to 5e13vg per cell stack, and titers of 5e12vg/ml to 1e14 vg/ml, depending on both the capsid and vector cassette packaged. Cost includes AAV cap plasmid (unless novel capsid, then plasmid supplied by client) and pAd helper plasmid. Typical turnaround for vector modification is 4-6 weeks from receipt of client plasmidSelect Capsid: AAV1 AAV2 AAV2(Y444F) AAV2-TrpYF, aka Y444F+Y500F+Y730F AAV2(quadY-F+T-V), aka Y272F+Y444F+Y500F+Y730F+T491V AAV2(4pMut)ΔHS AAV5 AAV6 AAV6-TrpMut, aka Y705F+Y731F+T492V AAV8 AAV8(Y733F) AAV8-TrpMut, aka Y447F+Y733F+T494V AAV9 AAV44.9 AAV44.9 (E531D) Select AAV Vector Plasmid Vector Core reporter constructs Client provided AAV vector plasmid Select Reporter Construct pTR-UF5 (CMV-GFP) pTR-UF11 (CBA-GFP) CBA-mCherry with WPRE CBA-Luciferase(ff) Self-complementary CBA-mCherry Self-complementary CBA-GFP Note: we require 650ug of AAV vector plasmid DNA per Cell StackName of AAV Vector Plasmid Gene of Interest Select how many Cell Stacks you would likeFirst Cell Stack is $2,630. Preparations requiring up to 4 additional Cell Stacks are discounted at a rate of $1,742 per additional Cell Stack. Please contact the Vector Core if you suspect your request will exceed a total of 5 Cell Stacks. If ordering more than one cell stack, please note that they will be pooled together.1 Cell stack2 Cell stacks3 Cell stacks4 Cell stacks5 Cell stacksSelect Additional AAV Purification (Optional) - $700 None Affinity purification with AAVX Affinity purification with CaptureSelect AAV8 Affinity purification with CaptureSelect AAV9 *Please inquire if additional processing for enhanced for full capsids is desired. (sboye@ufl.edu) Select AAV BufferUnless specified, our final excipient buffers will contain 0.001% Pluronic F68. Pluronic is a widely used non-ionic surfactant added to prevent aggregation during concentration and storage. *Deviations from standard buffers are acceptable but may require a brief consult. Balanced Salt Solution plus Pluronic (BSS-P) Lactated Ringers Solution plus Pluronic (LRS-P) TMN200-P Phosphate Buffered Saline plus Pluronic (PBS-P) Other Please specify the AAV Buffer you would likeCompatibility of the buffers listed has been confirmed, selection of an alternate buffer is at the requester's risk Select Additional Testing of Final AAV Vector Endotoxin testing (with Charles River Endosafe-PTS) Please include any special requests or additional commentsWould you like to order a TENTH vector? Yes No Vector Production #10AAV Capsid Selection Price: Includes transfection of a 10 layer cell stack of HEK293 cells, purification of AAV by iodixanol gradient, ion exchange chromatography (if applicable capsid type) and buffer exchange/concentration of final vector into user defined buffer and testing of final AAV vector for vector genome titer (qPCR) and purity/identity (PAGE). Typical yield of AAV is 5e12vg to 5e13vg per cell stack, and titers of 5e12vg/ml to 1e14 vg/ml, depending on both the capsid and vector cassette packaged. Cost includes AAV cap plasmid (unless novel capsid, then plasmid supplied by client) and pAd helper plasmid. Typical turnaround for vector modification is 4-6 weeks from receipt of client plasmidSelect Capsid: AAV1 AAV2 AAV2(Y444F) AAV2-TrpYF, aka Y444F+Y500F+Y730F AAV2(quadY-F+T-V), aka Y272F+Y444F+Y500F+Y730F+T491V AAV2(4pMut)ΔHS AAV5 AAV6 AAV6-TrpMut, aka Y705F+Y731F+T492V AAV8 AAV8(Y733F) AAV8-TrpMut, aka Y447F+Y733F+T494V AAV9 AAV44.9 AAV44.9 (E531D) Select AAV Vector Plasmid Vector Core reporter constructs Client provided AAV vector plasmid Select Reporter Construct pTR-UF5 (CMV-GFP) pTR-UF11 (CBA-GFP) CBA-mCherry with WPRE CBA-Luciferase(ff) Self-complementary CBA-mCherry Self-complementary CBA-GFP Note: we require 650ug of AAV vector plasmid DNA per Cell StackName of AAV Vector Plasmid Gene of Interest Select how many Cell Stacks you would likeFirst Cell Stack is $2,630. Preparations requiring up to 4 additional Cell Stacks are discounted at a rate of $1,742 per additional Cell Stack. Please contact the Vector Core if you suspect your request will exceed a total of 5 Cell Stacks. If ordering more than one cell stack, please note that they will be pooled together.1 Cell stack2 Cell stacks3 Cell stacks4 Cell stacks5 Cell stacksSelect Additional AAV Purification (Optional) - $700 None Affinity purification with AAVX Affinity purification with CaptureSelect AAV8 Affinity purification with CaptureSelect AAV9 *Please inquire if additional processing for enhanced for full capsids is desired. (sboye@ufl.edu) Select AAV BufferUnless specified, our final excipient buffers will contain 0.001% Pluronic F68. Pluronic is a widely used non-ionic surfactant added to prevent aggregation during concentration and storage. *Deviations from standard buffers are acceptable but may require a brief consult. Balanced Salt Solution plus Pluronic (BSS-P) Lactated Ringers Solution plus Pluronic (LRS-P) TMN200-P Phosphate Buffered Saline plus Pluronic (PBS-P) Other Please specify the AAV Buffer you would likeCompatibility of the buffers listed has been confirmed, selection of an alternate buffer is at the requester's risk Select Additional Testing of Final AAV Vector Endotoxin testing (with Charles River Endosafe-PTS) Please include any special requests or additional commentsRunning Total: Contact InformationName of PI* Institution* Requested By* Contact's Email* Contact's Phone NumberAddress Street Address Address Line 2 City State / Province / Region ZIP / Postal Code AfghanistanAlbaniaAlgeriaAmerican SamoaAndorraAngolaAnguillaAntarcticaAntigua and BarbudaArgentinaArmeniaArubaAustraliaAustriaAzerbaijanBahamasBahrainBangladeshBarbadosBelarusBelgiumBelizeBeninBermudaBhutanBoliviaBonaire, Sint Eustatius and SabaBosnia and HerzegovinaBotswanaBouvet IslandBrazilBritish Indian Ocean TerritoryBrunei DarussalamBulgariaBurkina FasoBurundiCabo VerdeCambodiaCameroonCanadaCayman IslandsCentral African RepublicChadChileChinaChristmas IslandCocos IslandsColombiaComorosCongoCongo, Democratic Republic of theCook IslandsCosta RicaCroatiaCubaCuraçaoCyprusCzechiaCôte d'IvoireDenmarkDjiboutiDominicaDominican RepublicEcuadorEgyptEl SalvadorEquatorial GuineaEritreaEstoniaEswatiniEthiopiaFalkland IslandsFaroe IslandsFijiFinlandFranceFrench GuianaFrench PolynesiaFrench Southern TerritoriesGabonGambiaGeorgiaGermanyGhanaGibraltarGreeceGreenlandGrenadaGuadeloupeGuamGuatemalaGuernseyGuineaGuinea-BissauGuyanaHaitiHeard Island and McDonald IslandsHoly SeeHondurasHong KongHungaryIcelandIndiaIndonesiaIranIraqIrelandIsle of ManIsraelItalyJamaicaJapanJerseyJordanKazakhstanKenyaKiribatiKorea, Democratic People's Republic ofKorea, Republic ofKuwaitKyrgyzstanLao People's Democratic RepublicLatviaLebanonLesothoLiberiaLibyaLiechtensteinLithuaniaLuxembourgMacaoMadagascarMalawiMalaysiaMaldivesMaliMaltaMarshall IslandsMartiniqueMauritaniaMauritiusMayotteMexicoMicronesiaMoldovaMonacoMongoliaMontenegroMontserratMoroccoMozambiqueMyanmarNamibiaNauruNepalNetherlandsNew CaledoniaNew ZealandNicaraguaNigerNigeriaNiueNorfolk IslandNorth MacedoniaNorthern Mariana IslandsNorwayOmanPakistanPalauPalestine, State ofPanamaPapua New GuineaParaguayPeruPhilippinesPitcairnPolandPortugalPuerto RicoQatarRomaniaRussian FederationRwandaRéunionSaint BarthélemySaint Helena, Ascension and Tristan da CunhaSaint Kitts and NevisSaint LuciaSaint MartinSaint Pierre and MiquelonSaint Vincent and the GrenadinesSamoaSan MarinoSao Tome and PrincipeSaudi ArabiaSenegalSerbiaSeychellesSierra LeoneSingaporeSint MaartenSlovakiaSloveniaSolomon IslandsSomaliaSouth AfricaSouth Georgia and the South Sandwich IslandsSouth SudanSpainSri LankaSudanSurinameSvalbard and Jan MayenSwedenSwitzerlandSyria Arab RepublicTaiwanTajikistanTanzania, the United Republic ofThailandTimor-LesteTogoTokelauTongaTrinidad and TobagoTunisiaTurkmenistanTurks and Caicos IslandsTuvaluTürkiyeUS Minor Outlying IslandsUgandaUkraineUnited Arab EmiratesUnited KingdomUnited StatesUruguayUzbekistanVanuatuVenezuelaViet NamVirgin Islands, BritishVirgin Islands, U.S.Wallis and FutunaWestern SaharaYemenZambiaZimbabweÅland Islands Country Final TotalPlease note this is just an estimate. 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