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Powell Gene Therapy Center Department of Pediatrics | College of Medicine
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Vector Core Overview

Enhanced for Full Capsids Standards

AAV-CBA-GFP (UFII) Enhanced for Full Capsids
Protein gel analysis of AAV‑CBA‑GFP (UFII) full‑capsid preparations. VP1, VP2, and VP3 capsid proteins are shown for AAV2, AAV5, AAV8, and AAV9 at 1×10¹¹ vg per lane (ddPCR‑quantified). This analysis supports the sample’s high capsid quality and purity.

To order Enhanced for Full Capsid Standards please e-mail vectorcore-quoterequest@peds.ufl.edu with the serotype and aliquot size and number requested, along with your contact details (name, institution, address, and telephone number). We will send you a quote in reply.

  • Enhanced to greater than 98.63% full capsids
  • Equivalent vector genome as ATCC reference standard material (RSM) VR-1616.
  • Useful as a standard for capsid and vector genome tittering.
  • Tittered by ddPCR.
  • 25μL aliquots $150 for academic, $210 for commercial; 100μL aliquots $600 for academics, $840 for commercial

Material Descriptions

Cryo‑EM image of AAV particles used to assess capsid purity
Cryogenic electron microscopy (cryo‑EM) image of AAV2 capsids used to assess capsid purity, showing a high proportion of full capsids (99.24%).
Protein gel showing VP1–VP3 bands; AAV2 column highlighted

AAV2-CBA-GFP (UF11)

Name: EFC Standard AAV2-CBA-GFP (UF11)
Lot number: 082522
Quantity: 5.0 ml
Viral titer in vector genomes: 4.40E+12 vg/ml
Storage buffer: TMN200-P
Method of purification: Iodixanol gradient, FPLC affinity chromatography (AAV-X), Cesium Chloride, Dialysis

Specifications:

  • Viral titer
    • Test method: ddPCR
    • Determination: Vector genomes
    • Result: 4.40×10¹² vg/ml
  • Stability
    • Stable indefinitely when stored at −80°C
  • Grade
    • Highly enriched for full capsid standard
  • Capsid Purity – % Full Capsids
    • Test method: Cryogenic electron microscopy (cryo‑EM)
    • Result: 99.24% full capsids
Cryo‑EM image of AAV5 particles used to assess capsid purity
Cryogenic electron microscopy (cryo‑EM) image of AAV5 capsids used for capsid‑purity assessment. The sample shows a high proportion of full capsids consistent with its reported purity

AAV5-CBA-GFP (UF11) 

Protein gel showing VP1–VP3 bands; AAV5 column highlighted

Name: EFC Standard AAV5-CBA-GFP
Lot number: 082422
Quantity: 3.0 ml
Viral titer in vector genomes: 6.34E+12 vg/ml
Storage buffer: PBS-P
Method of purification: Iodixanol gradient, Anion exchange chromatography (Q), Centrifugal concentrator, Cesium Chloride, Dialysis

Specifications:

  • Viral titer
    • Test method: ddPCR
    • Determination: Vector genomes
    • Result: 6.34E+12 vg/ml
  • Stability
    • Stable indefinitely when stored at -60C
  • Grade
    • Highly enriched for full capsid standard
  • Capsid Purity – % Full Capsids
    • Test method: Cryogenic electron microscopy (cryo-EM)
    • Result: 98.63% Full Capsid
Cryo‑EM image showing AAV particles dispersed across the grid
Figure: Cryogenic electron microscopy (cryo‑EM) image of AAV capsids at ~100 nm scale, used to evaluate capsid morphology and purity
Protein gel showing VP1–VP3 bands; AAV58 column highlighted

AAV8-CBA-GFP (UF11)

Name: EFC Standard AAV8-CBA-GFP (UF11)
Lot number: 040522
Quantity: 1.5 ml
Viral titer in vector genomes: 6.91E+12 vg/ml
Storage buffer: PBS-P
Method of purification: Iodixanol gradient, FPLC affinity chromatography (AAV-X), Centrifugal concentrator, Cesium Chloride, TFF, Centrifugal concentrator

Specifications:

  • Viral titer
    • Test method: ddPCR
    • Determination: Vector genomes
    • Result: 6.91E+12 vg/ml
  • Stability
    • Stable indefinitely when stored at -60C
  • Grade
    • Highly enriched for full capsid standard
  • Capsid Purity – % Full Capsids
    • Test method: Cryogenic electron microscopy (cryo-EM)
    • Result: 99.28% Full Capsid
Cryo‑EM image showing a dense field of AAV particles.
Figure: Cryogenic electron microscopy (cryo‑EM) image showing a densely packed field of AAV capsids at ~100 nm scale, used to evaluate capsid morphology and purity.
Protein gel showing VP1–VP3 bands; AAV8 column highlighted

AAV9-CBA-GFP (UF11)

Name: EFC Standard AAV9-CBA-GFP (UF11)
Lot number: 090222
Quantity: 4.5 ml
Viral titer in vector genomes: 5.90E+12 vg/ml
Storage buffer: PBS-P
Method of purification: Iodixanol gradient, FPLC affinity chromatography (Capture Select-9), Cesium Chloride, Dialysis

Specifications:

  • Viral titer
    • Test method: ddPCR
    • Determination: Vector genomes
    • Result: 5.90E+12 vg/ml
  • Stability
    • Stable indefinitely when stored at -60C
  • Grade
    • Highly enriched for full capsid standard
  • Capsid Purity – % Full Capsids
    • Test method: Cryogenic electron microscopy (cryo-EM)
    • Result: 99.38% Full Capsid