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UF Health University of Florida
Powell Gene Therapy Center Department of Pediatrics | College of Medicine
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Vector Core Overview

Reporter Construct Maps

pTR-UF5 (CMV-GFP)

Plasmid map of pTR-UF5 (CMV-GFP), 6,253 bp, showing GFP, CMV promoter, AmpR, NeoR, and regulatory elements.
Circular plasmid map of the pTR-UF5 (CMV-GFP) vector (6,253 bp) displaying key genetic elements, including the CMV promoter, GFP reporter gene, AmpR and NeoR antibiotic resistance markers, SV40 regulatory sequences, and replication origins.

pTR-UF11 (CBA-GFP)

Plasmid map of pTR-UF11 (CBA-GFP), 7,199 bp, showing GFP, CBA promoter, AmpR, NeoR, and enhancers.
Circular plasmid map of the pTR-UF11 (CBA-GFP) vector (7,199 bp) showing major genetic elements, including the CMV immediate-early enhancer, chicken β-actin promoter (CBA), exon 1, intron, GFP reporter gene, AmpR and NeoR resistance markers, SV40 poly(A), and replication origins.

CBA-mCherry with WPRE

Plasmid map of pTR-CBA-mCher-mcs-WPRE, 6,499 bp, showing mCherry, CBA promoter, and WPRE.
Circular plasmid map of the pTR-CBA-mCher-mcs-WPRE vector (6,499 bp) showing the CMV immediate-early enhancer, chicken β-actin (CBA) promoter, exon 1, intron, mCherry fluorescent reporter gene, multiple cloning site (MCS) for barcode insertion, WPRE sequence, SV40 poly(A) signal, AmpR marker, and replication origins.

CBA-Luciferase(ff)

Plasmid map of pTR-SB-smCBA-LUC-V2, 6,084 bp, showing luciferase gene, CBA promoter, and AmpR.
Circular plasmid map of the pTR-SB-smCBA-LUC-V2 vector (6,084 bp) showing the CMV immediate-early enhancer, chicken β-actin (CBA) promoter, exons 1 and 2, chimeric intron, firefly luciferase (LUC) reporter gene, SV40 poly(A) signal, AmpR antibiotic resistance marker, terminal repeats (TR), and replication origins.

Self-complementary CBA-mCherry

Plasmid map of Sc-smCBA-mCherry, 5,861 bp, showing mCherry, CBA promoter, AmpR, and TR elements.
Circular plasmid map of the Sc-smCBA-mCherry vector (5,861 bp) showing the CMV immediate-early enhancer, chicken β-actin (CBA) promoter, exon 1, chimeric intron, mCherry fluorescent reporter gene, bGH poly(A) signal, AmpR antibiotic resistance marker, and terminal repeat (TR) sequences, including a self-complementary TR element.

Self-complementary CBA-GFP

Plasmid map of SC-smCBA-hGFP, 6,087 bp, showing GFP, CBA promoter, AmpR, and regulatory elements.
Circular plasmid map of the SC-smCBA-hGFP vector (6,087 bp) showing the CMV immediate-early enhancer, chicken β-actin (CBA) promoter, exons 1 and 2, chimeric intron, GFP reporter gene, SV40 and bGH poly(A) signals, AmpR antibiotic resistance marker, and terminal repeat (TR) sequences, including a self-complementary TR element.

pTR-FLEX td-Tomato

Plasmid map of pAAV-FLEX-CBA-tdTomato, 6,469 bp, showing tdTomato, CBA promoter, and lox sites.
Circular plasmid map of the pAAV-FLEX-CBA-tdTomato vector (6,469 bp) showing the chicken β-actin (CBA) promoter, tdTomato fluorescent reporter gene, loxP and lox2272 recombination sites in a FLEX configuration, WPRE element, SV40 poly(A) signal, AmpR antibiotic resistance gene and promoter, replication origins, and AAV inverted terminal repeats (ITRs).

pTR-CBA-CRE-P2A-GFP

Plasmid map of pTR-CBA-Cre-P2A-GFP, 6,990 bp, showing Cre, GFP, CBA promoter, and AmpR.
Circular plasmid map of the pTR-CBA-Cre-P2A-GFP vector (6,990 bp) showing the CMV immediate-early enhancer, chicken β-actin (CBA) promoter, exon 1, intron, Cre recombinase gene, P2A self-cleaving peptide sequence, GFP reporter gene, bGH poly(A) signal, AmpR antibiotic resistance marker, ColE1 and f1 replication origins, and terminal repeat (TR) sequences.